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united states cat 12721  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc united states cat 12721
    United States Cat 12721, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/united+states+cat+12721/NRF2+XP+Rabbit+mAb/pmc13043341-113-40-46
    Average 98 stars, based on 1562 article reviews
    united states cat 12721 - by Bioz Stars, 2026-09
    98/100 stars

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    Blocking Assay:

    Article Title: Short-term cigarette smoke exposure aggravates oxidative stress and airway inflammation induced by lipopolysaccharides
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against NOX2 (gp91^phox) (1:2000, BD Biosciences, United States, cat# 611415), HO-1 (1:2000, Abcam, United States, cat# ab13248), nuclear factor erythroid 2-related factor 2 (Nrf2) (1:1000, Cell Signaling Technology, United States, cat# 12721), caspase-3 (1:1000, Cell Signaling Technology, United States, cat# 9662), cleaved caspase-3 (1:1000, Cell Signaling Technology, United States, cat# 9661), IκBα (1:1000, Cell Signaling Technology, United States, cat# 4814), phosphorylated IκBα (p-IκBα) (1:1000, Cell Signaling Technology, United States, cat# 9246), GAPDH (1:1000, Cell Signaling Technology, United States, cat# 2118), TGFβ-1 (1:1000, Santa Cruz Biotechnology, United States, cat# sc-52893), and β-actin (1:1000, Santa Cruz Biotechnology, United States, cat# sc-81178). .. Following incubation with HRP-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibodies, immunoreactive bands were visualized using the Gel DocTM EZ imaging system (Bio-Rad, United States). β-actin or GAPDH was used as the housekeeping protein.

    Incubation:

    Article Title: Short-term cigarette smoke exposure aggravates oxidative stress and airway inflammation induced by lipopolysaccharides
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against NOX2 (gp91^phox) (1:2000, BD Biosciences, United States, cat# 611415), HO-1 (1:2000, Abcam, United States, cat# ab13248), nuclear factor erythroid 2-related factor 2 (Nrf2) (1:1000, Cell Signaling Technology, United States, cat# 12721), caspase-3 (1:1000, Cell Signaling Technology, United States, cat# 9662), cleaved caspase-3 (1:1000, Cell Signaling Technology, United States, cat# 9661), IκBα (1:1000, Cell Signaling Technology, United States, cat# 4814), phosphorylated IκBα (p-IκBα) (1:1000, Cell Signaling Technology, United States, cat# 9246), GAPDH (1:1000, Cell Signaling Technology, United States, cat# 2118), TGFβ-1 (1:1000, Santa Cruz Biotechnology, United States, cat# sc-52893), and β-actin (1:1000, Santa Cruz Biotechnology, United States, cat# sc-81178). .. Following incubation with HRP-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibodies, immunoreactive bands were visualized using the Gel DocTM EZ imaging system (Bio-Rad, United States). β-actin or GAPDH was used as the housekeeping protein.



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    united states cat 12721  (Cell Signaling Technology Inc)
    98
    Cell Signaling Technology Inc united states cat 12721
    United States Cat 12721, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/united+states+cat+12721/NRF2+XP+Rabbit+mAb/pmc13043341-113-40-46
    Average 98 stars, based on 1 article reviews
    united states cat 12721 - by Bioz Stars, 2026-09
    98/100 stars
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